Journal: STAR Protocols

Article Title: Protocol for morphogen-guided differentiation of brain cell types using human induced pluripotent stem cells

doi: 10.1016/j.xpro.2025.104339

Figure Lengend Snippet: Representative immunofluorescent images of human microglia on Day 38 Top to bottom: PU.1, CX3CR1, TMEM119, IBA1, P2RY12, TREM2 positive human microglial markers showing microglial maturation. Scale bar: 50 μm.

Article Snippet: CX3CR1 (1:500) , BioRad , Cat# AHP1589, RRID: AB_2087421.

Techniques:

Journal: Oncology reports

Article Title: Overexpression of CX3CR1 is associated with cellular metastasis, proliferation and survival in gastric cancer.

doi: 10.3892/or.2014.3645

Figure Lengend Snippet: Figure 1. Expression of CX3CR1 in gastric cancer and non-neoplastic gastric tissues. (A) The protein levels of CX3CR1 in the gastric cancer and the non-neoplastic gastric tissues were detected by immunohistochemistry (magnification, x400). (B) The mRNA levels of CX3CR1 in the gastric cancer and non-neoplastic gastric tissues were detected by quantative real‑time PCR. **P<0.01.

Article Snippet: Sections were incubated overnight with the diluted rabbit anti-human CX3CR1 antibody (origene) at 1:100 in PBS in a humidified chamber at 4 ̊C.

Techniques: Expressing, Immunohistochemistry, Real-time Polymerase Chain Reaction

Journal: Oncology reports

Article Title: Overexpression of CX3CR1 is associated with cellular metastasis, proliferation and survival in gastric cancer.

doi: 10.3892/or.2014.3645

Figure Lengend Snippet: Figure 2. Expression of CX3CR1 in gastric cancer cell lines and a gastric epithelial cell line. The expression of CX3CR1 in three gastric cancer cell lines (MKN- 28, SGC-7901 and MKN-45) and a gastric epithelial cell line (GES-1) was detected by (A) immunocytochemistry (magnification, x400), (B) quantative real-time PCR (NS, no significance; **P<0.01) and (C) western blotting.

Article Snippet: Sections were incubated overnight with the diluted rabbit anti-human CX3CR1 antibody (origene) at 1:100 in PBS in a humidified chamber at 4 ̊C.

Techniques: Expressing, Immunocytochemistry, Real-time Polymerase Chain Reaction, Western Blot

Journal: Oncology reports

Article Title: Overexpression of CX3CR1 is associated with cellular metastasis, proliferation and survival in gastric cancer.

doi: 10.3892/or.2014.3645

Figure Lengend Snippet: Figure 3. Identification of stable CX3CR1-overexpressing or -knockdown cells. Three gastric cancer cell lines (MKN-28, SGC-7901 and MKN-45) and a gastric epithelial cell line (GES-1) were transfected with CX3CR1 cDNA or CX3CR1 short hairpin (sh)RNA, and then the CX3CR1 level was assessed in the stable transfected cells as detected by (A) fluorescence microscopy, (B) western blotting and (C) quantative real-time PCR. *P<0.05; **P<0.01.

Article Snippet: Sections were incubated overnight with the diluted rabbit anti-human CX3CR1 antibody (origene) at 1:100 in PBS in a humidified chamber at 4 ̊C.

Techniques: Knockdown, Transfection, Fluorescence, Microscopy, Western Blot, Real-time Polymerase Chain Reaction

Journal: Oncology reports

Article Title: Overexpression of CX3CR1 is associated with cellular metastasis, proliferation and survival in gastric cancer.

doi: 10.3892/or.2014.3645

Figure Lengend Snippet: Figure 4. CX3CR1/CX3CL1 mediates the migration and invasion of gastric cancer and gastric epithelial cells. The migration and invasion abilities of the CX3CR1-overexpressing and -knockdown cells were assessed by Transwell chamber. The cells on the lower side of the membrane were counted by microscopy from 5 fields at a x200 magnification. NS, no significance; *P<0.05; **P<0.01.

Article Snippet: Sections were incubated overnight with the diluted rabbit anti-human CX3CR1 antibody (origene) at 1:100 in PBS in a humidified chamber at 4 ̊C.

Techniques: Migration, Knockdown, Membrane, Microscopy

Journal: Oncology reports

Article Title: Overexpression of CX3CR1 is associated with cellular metastasis, proliferation and survival in gastric cancer.

doi: 10.3892/or.2014.3645

Figure Lengend Snippet: Figure 5. Effect of the CX3CR1/CX3CL1 axis on the proliferation of gastric cancer and gastric epithelial cells. The CCK-8 assay was used to detect the proliferation of the CX3CR1-overexpressing and -knockdown cells.

Article Snippet: Sections were incubated overnight with the diluted rabbit anti-human CX3CR1 antibody (origene) at 1:100 in PBS in a humidified chamber at 4 ̊C.

Techniques: CCK-8 Assay, Knockdown

Journal: Oncology reports

Article Title: Overexpression of CX3CR1 is associated with cellular metastasis, proliferation and survival in gastric cancer.

doi: 10.3892/or.2014.3645

Figure Lengend Snippet: Figure 6. Effect of the CX3CR1/CX3CL1 axis on the apoptosis of gastric cancer and gastric epithelial cells. The percentages of Annexin V-positive cells in the CX3CR1-overexpressing and -knockdown cells cultured in serum‑free medium for 24 h with CX3CL1 are shown. *P<0.05; **P<0.01.

Article Snippet: Sections were incubated overnight with the diluted rabbit anti-human CX3CR1 antibody (origene) at 1:100 in PBS in a humidified chamber at 4 ̊C.

Techniques: Knockdown, Cell Culture

Journal: Oncology reports

Article Title: Overexpression of CX3CR1 is associated with cellular metastasis, proliferation and survival in gastric cancer.

doi: 10.3892/or.2014.3645

Figure Lengend Snippet: Figure 7. Effect of the CX3CR1/CX3CL1 axis on the Akt signaling pathways. Activation of Akt through Ser-473 was determined by western blot analysis of CX3CR1-overexpressing and -knockdown cells after pre-incubation with 200 ng/ml CX3CL1 for 24 h. (A) Representative images and (B) statistical analysis of the results are shown. NS, no significance; *P<0.05; **P<0.01.

Article Snippet: Sections were incubated overnight with the diluted rabbit anti-human CX3CR1 antibody (origene) at 1:100 in PBS in a humidified chamber at 4 ̊C.

Techniques: Protein-Protein interactions, Activation Assay, Western Blot, Knockdown, Incubation